Sodium, potassium, chloride, and water in frog gastric mucosa

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Abstract
In fresh mucosas Na is 45–52, K 52–60, and Cl 39–41 mEq/kg. In mucosas incubated 4 hr at 25 C in salt solution, equilibrium volume of radio-iodinated serum albumin (RISA) is 231 ± 7 ml/kg. Other volumes are: inulin 383 ± 14, mannitol 515 ± 22, arabinose 550 ± 22. During incubation with or without 0.1 mm histamine, mucosal Na remains constant at 72 ± 2, K rises from 51 ± 1 to 63 ± 1, and Cl falls from 50 ± 3 to 32 ± 4 mEq/kg. With 0.1 mm 2,4-dinitrophenol, Na and Cl rise while K falls. When [Na]0 is constant, mucosal Na remains constant, but K and Cl are direct linear functions of [K]0 and [Cl]0. When [K]0 replaces [Na]0 mole for mole, mucosal Na falls and K rises. Mucosas stripped of surface epithelial cells have the following volumes: RISA 336 ± 13, inulin 500 ± 23, arabinose 629 ± 38 ml/kg. On incubation Na and K are approximately constant, but Cl falls.