Intramolecular Dimers: A New Design Strategy for Fluorescence‐Quenched Probes
- 11 July 2003
- journal article
- concept
- Published by Wiley in Chemistry – A European Journal
- Vol. 9 (15) , 3466-3471
- https://doi.org/10.1002/chem.200304941
Abstract
Fluorogenic probes dual-labeled with reporter and quencher dyes use a change in fluorescence to monitor biochemical events (e.g., substrate binding or enzyme digestion). Such events change the reporter–quencher distance, which affects fluorescence. Recently, it is has been shown that static quenching through intramolecular dimers is an important mechanism that can sometimes be more efficient than Förster resonance energy transfer (FRET).Keywords
This publication has 43 references indexed in Scilit:
- Application of Micro Arrayed Compound Screening (μARCS) to Identify Inhibitors of Caspase-3SLAS Discovery, 2002
- Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assaysJournal of Molecular Endocrinology, 2000
- Intramolecular Excitonic Dimers in Protease Substrates: Modification of the Backbone Moiety To Probe the H-Dimer StructureThe Journal of Physical Chemistry B, 1998
- Structural Characteristics of Fluorophores That Form Intramolecular H-Type Dimers in a Protease SubstrateThe Journal of Physical Chemistry B, 1997
- Photophysics of Cyanine Dyes: Subnanosecond Relaxation Dynamics in Monomers, Dimers, and H- and J-Aggregates in SolutionThe Journal of Physical Chemistry B, 1997
- Molecular Beacons: Probes that Fluoresce upon HybridizationNature Biotechnology, 1996
- Donor-Acceptor Distance Distributions in a Double-Labeled Fluorescent Oligonucleotide Both as a Single Strand and in DuplexesBiochemistry, 1995
- Antibody-Mediated Fluorescence Enhancement Based on Shifting the Intramolecular Dimer .dblarw. Monomer Equilibrium of Fluorescent DyesAnalytical Chemistry, 1994
- Zwischenmolekulare Energiewanderung und FluoreszenzAnnalen der Physik, 1948
- Spectral Absorption and Fluorescence of Dyes in the Molecular StateNature, 1936