CHEMILUMINESCENCE FROM AUTOXIDIZING 6‐HYDROXYDOPAMINE: THE INVOLVEMENT OF ACTIVATED FORMS OF OXYGEN

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Abstract
Abstract—The autoxidation of the catecholamine neurotoxin 6‐hydroxydopamine (20 μM) gave rise to a chemiluminescence which was greatly stimulated by FeSO4(20 μM) or by hydrogen peroxide addition (20 μMto 2 mM). The luminescence of both 6‐hydroxydopamine alone or 6‐hydroxydopamine plus hydrogen peroxide was strongly inhibited by catalase and by superoxide dismutase (both at 10 μg/m/); bovine serum albumin at 10 μg/m/ had no inhibitory effect. The luminescence was also strongly inhibited by several potent hydroxyl radical trapping agents and also by low concentrations of the1O2quencher DABCO (l,4‐diazabicyclo‐2.2.2.‐octane). Chemiluminescence was greatly enhanced in D2O, a solvent in which1O2has a prolonged lifetime. These data demonstrate the involvement of hydrogen peroxide, the superoxide radical and the hydroxyl radical in the chemiluminescence. The data are also consistent with some role for1O2.