Chromosome damage and SCE induced by the cytostatic factor homo‐aza‐steroidal ester of P‐bis (2‐chloro‐ethyl) amino phenyl acetic acid in CHO cells in culture

Abstract
The cytogenetic effects of homo-aza-steroidal ester (ASE) on Chinese hamster ovary (CHO) cells in vitro are reported. It was found that treatment of cells with a dose as low as 0.025 μg/ml of ASE causes a significant number of abnormal metaphases containing mostly chromatid-type aberrations. Similarly, significant frequencies of SCE were induced with only 0.075 μg/ml of ASE.