A mechanism for the transfer of the carboxyl-group from 1'-N -carboxybiotin to acceptor substrates by biotin-containing enzymes
- 29 October 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 163 (1) , 6-9
- https://doi.org/10.1016/0014-5793(83)81150-8
Abstract
Previous proposals for the mechanism by which biotin-dependent enzymes catalyse the transfer of the carboxyl group from 1'-N-carboxybiotin to acceptor molecules do not appear to be consistent with all of the experimental observations now available. We propose a multi-step mechanism in which (a) substrate and then carboxybiotin bind at the second partial reaction site, (b) a base positioned adjacent to the 3'-N of the carboxybiotin abstracts a proton from the 3'-N and (c) the resulting enolate ion and the acceptor substrate undergo a concerted reaction resulting in carboxyl-group transfer.Keywords
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