A CELL-LINE SECRETING STIMULATING FACTORS FOR CFU-GEMM CULTURE
- 1 January 1984
- journal article
- research article
- Vol. 64 (1) , 152-155
Abstract
The multipotent hemopoietic stem cell has fastidious growth requirements in vitro. Phytohemagglutinin-stimulated leukocyte conditioned medium [PHA-LCM] has been used to supply the undefined growth factors required for culture of the human multipotent hemopoietic progenitor. The use of medium conditioned by the bladder carcinoma cell line, 5637, to replace PHA-LCM in CFU-GEMM cultures was described and the properties of this conditioned medium closely mimic those of PHA-LCM in 2 separate CFU-GEMM culture systems.This publication has 9 references indexed in Scilit:
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