Conformational changes induced in DNA by in vitro reaction with N-hydroxy-N-2-aminofluorene
- 1 January 1979
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 6 (4) , 1683-1694
- https://doi.org/10.1093/nar/6.4.1683
Abstract
The conformation of DNA modified in vitro by the covalent binding of N-OH-AF was investigated by ultraviolet absorbance, circular dichroism and by radioimmunoassay using specific antibodies against Guo-AAF and nDNA-AAF. The results obtained by both physico-chemical and immunological methods are in agreement with a model involving destabilized regions in the double helical DNA around the carcinogen molecule in which, however, the -AF residues are stacked to the adjacent nucleotides. The RIA results show that the -AF residues are less accessible to antibodies in native than in denatured DNA-AF and thus suggest -AF residues partially buried in the interior of the DNA helix. The present model is compared to the one existing for DNA modified by reaction with N-AcO-AAF (DNA-AAF) (1,2).Keywords
This publication has 25 references indexed in Scilit:
- Antibodies to DNA modified by the carcinogen N -acetoxy-N -2-acetylaminofluoreneFEBS Letters, 1978
- Comparative orientation of the fluorene residue in native DNA modified by N-acetoxy-N-2-acetylaminofluorene and two 7-halogeno derivativesBiochemistry, 1976
- Dynamic structure of DNA modified with the carcinogen N-acetoxy-N-2-acetylaminofluoreneBiochemistry, 1974
- Physical basis of chemical carcinogenesis by N‐2‐fluorenylacetamide derivatives and analogsFEBS Letters, 1973
- Free energy of imperfect nucleic acid helicesJournal of Molecular Biology, 1973
- Changes of stability and conformation of DNA following the covalent binding of a carcinogenFEBS Letters, 1971
- Instability of fluorenylamine-substituted polynucleotides: Loss of carcinogen and production of an altered nucleic acidChemico-Biological Interactions, 1970
- On the mechanism of action of carcinogenic aromatic amines: I. Binding of 2-acetylaminofluorene and n-hydroxy-2-acetylaminofluorene to rat-liver nucleic acids in vivoChemico-Biological Interactions, 1969
- Nucleic Acid Guanine: Reaction with the Carcinogen N -Acetoxy-2-AcetylaminofluoreneScience, 1966
- On the interaction of N-2-fluorenylhydroxylamine with nucleic acids in, vitroBiochemical and Biophysical Research Communications, 1965