Candida albicans group A-specific soluble antigens demonstrated by quantitative immunoelectrophoresis

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Abstract
Soluble cytoplasmic extracts of C. albicans groups A and B were prepared and compared by quantitative immunoelectrophoresis experiments performed with a commercial anti-C. albicans group A immune serum. Although crossed immunoelectrophoresis, tandem crossed immunoelectrophoresis and line immunoelectrophoresis revealed many cross-reactions between the 2 groups, some components seemed to be specific to group A. The complexity of the extracts studied prevented the demonstration of specific constituents with these methods. Crossed-line immunoelectrophoresis with and without absorption of antibodies in situ was then used, and 4 specific antigens unique to group A cytoplasmic extract were demonstrated, 1 of which appeared to be quantitatively important. The value of various quantitative immunoelectrophoretic methods applied to complex antigenic preparations was discussed.