SPECIFIC SOLUBLE CELL-WALL ANTIGENS OF CANDIDA-TROPICALIS DEMONSTRATED BY CROSSED IMMUNOELECTROPHORESIS

  • 1 January 1979
    • journal article
    • research article
    • Vol. B130  (4) , 433-442
Abstract
Soluble cytoplasmic extracts of C. albicans and C. tropicalis were prepared and showed, respectively, 67 and 47 precipitates in crossed immunoelectrophoresis [CIE] performed with a commercial goat anti-C. albicans immune serum. Purified cell walls of C. tropicalis were prepared and corresponding antiserum was obtained in rabbits. Although important cross-reacting antigens [Ag] exist between soluble cytoplasmic extracts of C. albicans and C. tropicalis, the C. tropicalis cell wall antiserum precipitated Ag in CIE only in C. tropicalis soluble cytoplasmic extracts. C. albicans shared 70% of the Ag with C. tropicalis. Soluble cytoplasmic Ag prepared by homogenization of yeasts contain cell wall constituents; A specific immune serum for C. tropicalis could be prepared.