Improved Recovery of Rabies Virus from Cloned cDNA Using a Vaccinia Virus‐Free Reverse Genetics System
- 1 August 2003
- journal article
- Published by Wiley in Microbiology and Immunology
- Vol. 47 (8) , 613-617
- https://doi.org/10.1111/j.1348-0421.2003.tb03424.x
Abstract
To improve efficiency of recovery of rabies virus from cloned cDNA, we established a BHK cell clone that stably expresses T7 RNA polymerase, which we named BHK/T7-9. We also constructed new helper plasmids for expression of nucleoprotein and RNA polymerase of the RC-HL strain using the pTM1 plasmid vector, which makes the T7 RNA polymerase-transcripts from the plasmid cap-independent for translation. After co-transfection of these helper plasmids and the previously constructed full-length genome plasmid of the RC-HL strain to BHK/T7-9 cells, recombinant rabies virus was efficiently recovered from the cloned cDNA.Keywords
This publication has 19 references indexed in Scilit:
- An improved method for recovering rabies virus from cloned cDNAJournal of Virological Methods, 2002
- Reverse Genetics Demonstrates that Proteolytic Processing of the Ebola Virus Glycoprotein Is Not Essential for Replication in Cell CultureJournal of Virology, 2002
- Rescue of Rabies Virus from Cloned cDNA and Identification of the Pathogenicity-Related Gene: Glycoprotein Gene Is Associated with Virulence for Adult MiceJournal of Virology, 2001
- Recovery of Infectious Ebola Virus from Complementary DNA: RNA Editing of the GP Gene and Viral CytotoxicityScience, 2001
- Initiation of Sendai virus multiplication from transfected cDNA or RNA with negative or positive senseGenes to Cells, 1996
- Production of infectious human respiratory syncytial virus from cloned cDNA confirms an essential role for the transcription elongation factor from the 5' proximal open reading frame of the M2 mRNA in gene expression and provides a capability for vaccine development.Proceedings of the National Academy of Sciences, 1995
- Efficient recovery of infectious vesicular stomatitis virus entirely from cDNA clones.Proceedings of the National Academy of Sciences, 1995
- Recombinant vesicular stomatitis viruses from DNA.Proceedings of the National Academy of Sciences, 1995
- Linear and Conformation‐Dependent Antigenic Sites on the Nucleoprotein of Rabies VirusMicrobiology and Immunology, 1994
- New mammalian expression vectorsNature, 1990