Inhibition by l‐glutamine of the release of endothelium‐derived relaxing factor from cultured endothelial cells

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Abstract
L-Glutamine (0.02–2 mm) but not d-glutamine (0.2 mm and 2 mm) inhibited the release of endothelium-derived relaxing factor (EDRF) from bovine aortic endothelial cells cultured in the presence or absence of l-arginine. Inhibition was maximal at a concentration of 200 μm, and was reversed by l-arginine (50 μm) but not d-arginine (100 μm). l-Glutamic acid (2 mm) or ammonium chloride (1 mm), putative products of the metabolism of l-glutamine in endothelial cells, had no effect on EDRF release. l-Glutamine (0.2 mm and 2 mm) but not d-glutamine (2 mm), l-glutamic acid (2 mm) or ammonium chloride (1 mm) also inhibited the generation of l-arginine in endothelial cells. Thus, l-glutamine inhibits EDRF release by preventing the generation of l-arginine.