Determination of N,N'-Diphenyl-p-Phenylenediamine in Animal Tissues.

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Abstract
In connection with a study of replacement of Vit. E in vivo by certain synthetic antioxidants, liver uptake and intracellular distribution of the Vit. E-active compound N,N[image]-diphenyl-p-phenylenediamine (DPPD), following its administration to rats, has been determined by a method based on spectrophotometric measurement of the color developed by oxidation with ferric chloride in ethanol. Over half of the DPPD recovered was present in the soluble fraction of liver cells, one-quarter was associated with microsomes, and a small fraction recovered from mitochondria. This distribution pattern is similar to that reported for total lipids, and indicates that the intracellular location of DPPD may be passively determined by its lipid solubility. Alpha-tocopherol, on the other hand, was found predominantly in mitochondria.