Effect of Feeding N,N′-Diphexyl-Para-Phenylenediamine (DPPD) to Lactating Dairy Cows on the Production of Butterfat

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Abstract
Slight increases in the butterfat percentage were observed in 3 out of 4 experiments conducted at this station when N,N[image]-diphenyl-para-phenylenediamine (DPPD) was included in the ration of lactating cows for the purpose of inhibiting copper-induced oxidized milk flavor, suggesting that DPPD may affect lipid metabolism. However, no definite conclusions could be made, since feed intakes were not rigidly controlled or a system of equalized feeding was not used in the above-mentioned experiments. The present study was therefore initiated to determine the effect of feeding DPPD on the butterfat percentage of the milk of lactating dairy cows which were fed equivalent energy for maintenance and production. No discernible responses were observed for the supplementation with DPPD in lb. milk produced, the percentage butterfat or the daily yield of lb. of fat and FCM. Average daily intakes of estimated total digestible nutrients (TDN) and estimated net energy (ENE) were calculated using each cows actual feed intake and feed refusal and the proximate analyses for the particular week and were found not to differ between rations. The therms of ENE and lb. TDN required per lb. of FCM were derived and found to be the same whether DPPD was included in the ration or not. Concentration of DPPD averaged 12.7 [gamma]/100 ml of milk and on a per gram of fat basis 330 [gamma] [plus or minus] 0.37. This resulted in an apparent mammary transfer of 0.14% [plus or minus] 0.02 of the total DPPD fed.