Control of Precerebellar Neuron Development byOlig3bHLH Transcription Factor
Open Access
- 1 October 2008
- journal article
- research article
- Published by Society for Neuroscience in Journal of Neuroscience
- Vol. 28 (40) , 10124-10133
- https://doi.org/10.1523/jneurosci.3769-08.2008
Abstract
The rhombic lip (RL) is the neuroepithelium immediately adjacent to the roof plate of the fourth ventricle, and it gives rise to various brainstem and cerebellar cell types. Our study shows that the bHLH (basic helix-loop-helix) transcription factorOlig3is expressed in the progenitors of RL, and ablation ofOlig3significantly affects the development of RL. InOlig3−/− caudal RL, the expression level ofMath1in the dorsal interneuron 1 (dI1) domain is reduced, and the formation of four mossy-fiber nuclei is compromised; dI2–dI3 neurons are misspecified to dI4 interneurons, and the climbing-fiber neurons (inferior olive nucleus) are completely lost. In addition, the formation of brainstem (nor)adrenergic centers and first-order relay visceral sensory neurons is also dependent onOlig3. Therefore,Olig3plays an important role in the fate specification and differentiation of caudal RL-derived neurons.Keywords
This publication has 48 references indexed in Scilit:
- Cerebellar ‘transcriptome’ reveals cell-type and stage-specific expression during postnatal development and tumorigenesisMolecular and Cellular Neuroscience, 2006
- Hindbrain Rhombic Lip Is Comprised of Discrete Progenitor Cell Populations Allocated by Pax6Neuron, 2005
- Math1 Expression Redefines the Rhombic Lip Derivatives and Reveals Novel Lineages within the Brainstem and CerebellumNeuron, 2005
- Math1 Is Expressed in Temporally Discrete Pools of Cerebellar Rhombic-Lip Neural ProgenitorsNeuron, 2005
- Neuronal specification in the spinal cord: inductive signals and transcriptional codesNature Reviews Genetics, 2000
- Targeted deletion of the mouse POU domain gene Brn-3a causes selective loss of neurons in the brainstem and trigeminal ganglion, uncoordinated limb movement, and impaired suckling.Proceedings of the National Academy of Sciences, 1996
- The avian inferior olive derives from the alar neuroepithelium of the rhombomeres 7 and 8NeuroReport, 1996
- Brn-3.0 expression identifies early post-mitotic CNS neurons and sensory neural precursorsMechanisms of Development, 1995
- A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probesHistochemistry and Cell Biology, 1993
- Development of the precerebellar nuclei in the rat: IV. The anterior precerebellar extramural migratory stream and the nucleus reticularis tegmenti pontis and the basal pontine grayJournal of Comparative Neurology, 1987