Evidence for a single glycan moiety in rabbit serum transferrin and location of the glycan within the polypeptide chain

Abstract
The sequential removal of N‐acetylneuraminic acid from rabbit serum transferrin has been followed by urea‐polyacryl‐amide gel electrophoresis. The electrophoretic pattern is consistent with the presence of a single biantennary glycan chain. From the amino acid sequence of the carbohydrate‐containing cyanogen bromide fragment we have shown that the glycan is attached to an asparaginyl side chain at a position equivalent to residue 491 in the sequence of human serum transferrin.