Molecular Biological Characterization of a Highly Leukaemogenic Virus Isolated from the Mouse. IV. Viral Proteins
- 1 October 1984
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 65 (10) , 1791-1802
- https://doi.org/10.1099/0022-1317-65-10-1791
Abstract
The proteins of a highly leukemogenic murine virus (DMBA-LV) endogenous to the CFW/D mouse were characterized. This virus does not contain ecotropic, xenotropic or polytropic type C retroviruses capable of replicating in established tissue culture cell lines. Analysis of the viral proteins indicated that proteins characteristic of both type C and type B retroviruses were present. All the proteins characteristic of a competent, mature type B retrovirus, such as the murine mammary tumor virus, was present in the virions and were expressed at high levels in a virus-producing thymic lymphoma cell line. Type C internal structural proteins were detected in the virus and cells producing virus. Type C envelope protein was detected at very low levels in both the virions and the virus-producing tumor cell lines. Chymotryptic polypeptide profiles of this gp70 indicated similarity with the envelope glycoproteins characteristic of xenotropic and recombinant type C retroviruses.This publication has 20 references indexed in Scilit:
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