Isolation and Characterization of Parainfluenza 5 Virus from a Dog

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Abstract
A cytopathogenic agent was isolated in dog kidney cell cultures from pneumonic lung tissue of a 3 mo. old dog which died during an outbreak of respiratory disease. From results obtained in biological and physicochemical characterization, EM observation by negative staining and serum neutralization (SN) test against anti-SV5 serum, the isolate (DL-1 strain) was identified as parainfluenza virus type 5. In experimental infection with DL-1 strain, the pups inoculated intranasally, developed mild respiratory signs and SN antibody. In serologic survey of random street dogs in Kinki and Tokai districts [Japan], hemagglutination-inhibition (HI) antibody against the virus was not detected in sera collected from 1958-1975. HI antibody was found in 4 of 67 sera in 1976 and in 5 of 22 in 1977.