Enucleation and reconstruction of interferon-producing cells.

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Abstract
Enucleation of [mouse fibroblast] L cells leads to loss of the capacity to produce interferon, showing that the cell nucleus is essential for interferon formation. When cells were enucleated while interferon formation was proceeding, cytoplasts continued to synthesize interferon by a process shown to be protein synthesis, showing that the interferon mRNA leaves the nucleus after synthesis. Reconstructed cells were obtained by Sendai virus fusion of karyoplasts and cytoplasts. Such reconstructed cells produced at least as much interferon (43 interferon units/104 nucleated cells) as control cells (31 interferon units/104 nucleated cells).