Evidence for Autocrine Basis of Transformation in NIH-3T3 Cells Transfected with met/HGF Receptor Gene
- 1 January 1995
- journal article
- research article
- Published by Taylor & Francis in Growth Factors
- Vol. 12 (4) , 303-313
- https://doi.org/10.3109/08977199509028968
Abstract
NIH-3T3 cells transformed with met/HGF receptor gene proliferate in the absence of serum and growth factors. Immunocytochemical staining with anti-HGF antibody revealed intense HGF staining in the transfected cells. Additionally, these cells secrete bioactive HGF as evidenced by the ability of the conditioned media to stimulate met/HGF receptor phosphorylation in epithelial cells, and to promote migration of bovine adrenal capillary endothelial cells in a modified Boyden chamber assay. The migration of endothelial cells could be specifically inhibited by anti-HGF antibody but not by an irrelevant antibody. Suramin, a drug known to disrupt ligand-receptor interactions, inhibits the serum and growth-factor free proliferation, and the endogenous phosphorylation of met/HGF receptor in the transformed cells. Taken together, our data suggests an autocrine mode of transformation in NIH-3T3 cells transfected with met/HGF receptor gene.Keywords
This publication has 30 references indexed in Scilit:
- Angiogenesis.Journal of Biological Chemistry, 1992
- Growth Factors and CancerScience, 1991
- Identification of the Hepatocyte Growth Factor Receptor As the c- met Proto-Oncogene ProductScience, 1991
- Oncogenes and signal transductionCell, 1991
- Hepatocyte growth factor/hepatopoietin A stimulates the growth of rat kidney proximal tubule epithelial cells (RPTE), rat nonparenchymal liver cells, human melanoma cells, mouse keratinocytes and stimulates anchorage-independent growth of SV-40 transformed RPTEBiochemical and Biophysical Research Communications, 1991
- A tumor suppressor-dependent inhibitor of angiogenesis is immunologically and functionally indistinguishable from a fragment of thrombospondin.Proceedings of the National Academy of Sciences, 1990
- Overexpression of the human EGF receptor confers an EGF-dependent transformed phenotype to NIH 3T3 cellsCell, 1987
- Suramin inhibition of growth factor receptor binding and mitogenicity in AKR‐2B cellsJournal of Cellular Physiology, 1987
- cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell linesNature, 1986
- Bombesin-like peptides can function as autocrine growth factors in human small-cell lung cancerNature, 1985