High mobility group chromosomal proteins isolated from nuclei and cytosol of cultured hepatoma cells are similar

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Abstract
Using sequential chromatography on columns containing immobilized double-stranded DNA and single-stranded DNA, a protein was purified from the cytosol of an established line of cultured rat hepatoma cells that, by several criteria, is a high mobility group (HMG) protein. Analyses of DNA binding properties, electrophoretic mobilities, amino acid compositions and immunochemical reactivities reveal that the cytosolic protein is the same protein as HMG-1 isolated from the purified chromatin of the same cell line. Authentic HMG-1 appears to be at least partially responsible for the cytoplasmic fluorescence observed when mammalian cells are stained with fluorescent-labeled, affinity-purified antibodies against HMG-1. HMG-1 may possily shuttle between nucleus and cytoplasm, perhaps in response to the nucleus'' need for helix destabilizing proteins.

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