Cloning and sequencing of human λ immunoglobulin genes by the polymerase chain reaction

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Abstract
Universal oligonucleotide primers, designed for amplifying and sequencing genes encoding the rearranged human λ immunoglobulin variable region, were validated by amplification of the λ light chain genes from four human heterohybridoma cell lines and in the generation of a cDNA library of human Vλ sequences from Epstein‐Barr virus‐transformed human peripheral blood lymphocytes. This technique allows rapid cloning and sequencing of human immunoglobulin genes, and has potential applications in the rescue of unstable human antibody‐producing cell lines and in the production of human monoclonal antibodies.