Multiple conductance classes of mouse nicotinic acetylcholine receptors expressed in Xenopus oocytes.
- 1 March 1990
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 87 (6) , 2067-2071
- https://doi.org/10.1073/pnas.87.6.2067
Abstract
Acetylcholine receptor (AcChoR) subunit mRNAs transcribed from mouse BC3H-1 cDNAs were injected into Xenopus oocytes and the expressed AcChoR channels were examined by single channel recording. Injection of .alpha.-, .beta.-, .gamma.-, and .delta.-subunit mRNAs produced two predominant channel classes with conductances of .apprxeq.50 and .apprxeq.12pS, while infrequent openings of .apprxeq.25-pS channels were also observed. Injection of .alpha.-, .beta.-, and .gamma.-subunit mRNAs produced a single class of .apprxeq.12-pS AcChoR channels, which resembled the smallest conductance channels present in .alpha..beta..gamma..delta.-injected oocytes. Assembly of .delta.-less channels may thus explain the lowest conductance AcChoR channels in .alpha..beta..gamma..delta.-injected oocytes and might also account for similar channels that have been observed in vertebrate skeletal muscle.This publication has 27 references indexed in Scilit:
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