Expression of functional acetylcholine receptor from cloned cDNAs
- 1 February 1984
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 307 (5952) , 604-608
- https://doi.org/10.1038/307604a0
Abstract
The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the α-subunit is indispensable for α-bungarotoxin binding activity.Keywords
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