Characterisation of the Binding Sites of Anti-Parathyroid Hormone Antisera Using Synthetic Parathyroid Hormone Peptides

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Abstract
Four antisera raised against partly purified PTH preparations all showed a wide range of specificities when reacting with radioiodinated PTH peptides representing several different portions of the intact hormone sequence. In contrast, antisera raised against individual peptides were only able to cross-react with other peptides that contained all or part of their amino acid sequence in common. Cross-reacting peptides were seen to contain one or more amino acid residues having high interspecies variability in common. We have explained the antigenicity and cross-reactivity of the peptides on the basis of these common highly variable amino acid sequences. We have concluded that the selection of hormonal material in radio-immunoassays for PTH should be made on the basis of the highly variable amino acid residue content. This will allow a narowing of the assay specificities and permit detection of a desired region of the PTH hormone.