Radioimmunoassay for Albumin-Binding Sites Associated with HBsAg: Correlation of Results with the Presence of e-Antigen in Serum
- 1 January 1979
- journal article
- research article
- Published by S. Karger AG in Intervirology
- Vol. 11 (2) , 128-132
- https://doi.org/10.1159/000149024
Abstract
A radioimmunoassay for albumin-binding sites associated with hepatitis B surface antigen (HBsAg) is described. Polystyrene beads coated with glutaraldehyde-polymerized human serum albumin were incubated with diluted specimens of HBsAg-positive sera which contained either e-antigen (HBeAg) or anti-HBe or were negative for both HBeAg and anti-HBe as determined by rheophoresis. The quantity of HBsAg attached to the beads, determined by measuring the amount of 125I-labeled anti-HBs adsorbed during a subsequent incubation step, was related to the presence of HBeAg in the specimens.Keywords
This publication has 1 reference indexed in Scilit:
- Correlations between anti‐albumin antibodies and HBsAG in hepatic patientsJournal of Medical Virology, 1977