THERMODYNAMIC AND IMMUNOLOGICAL PROPERTIES OF A MONOCLONAL-ANTIBODY TO HUMAN-BLOOD GROUP-A

  • 1 January 1981
    • journal article
    • research article
    • Vol. 44  (3) , 549-554
Abstract
A murine anti-A monoclonal antibody was obtained by the hybridoma technique. This antibody, of an IgM nature, is capable of agglutinating A1, A2 and A3 red blood cells. Thermodynamic study confirmed its monoclonal character; its association constant is 1.6 .cntdot. 106 l/mol. The enthalpy change of the antigen/antibody reaction is null, which indicates the absence of the role of temperature on antibody fixation. This weak affinity makes it necessary to concentrate the supernatant so as to enable use of these reagents under the same conditions as those used at present.