Excess of Interfering over Infectious Particles in Herpes Simplex Virus Passaged at High m.o.i. and their Effect on Single-cell Survival

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Abstract
The capacity of herpes simplex virus to kill cells and to form both infectious centers and progeny virus was studied in [African green monkey kidney] cell culture. Two virus stocks were compared at various m.o.i. [multiplicity of infection]: a standard virus tock and a stock which contained interfering virus particles (I particles). The formation of infectious progeny virus and of infectious centers appeared to be reduced by the action of I particles. In the examined virus stock, I particles exceeded infectious particles by at least a factor of 5-10. More than 50% of cells which could be demonstrated to be hit by I particles survived and formed colonies.