Somatic embryogenesis and indole alkaloid production inCatharanthus roseus
- 1 January 2000
- journal article
- research article
- Published by Taylor & Francis in Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology
- Vol. 134 (2) , 179-184
- https://doi.org/10.1080/11263500012331358444
Abstract
A new protocol to obtain an embryogenic cell line from cultured seedling explants of Catharanthus roseus is described. In order to assess the relationship between tissue differentiation and secondary metabolite biosynthesis, the biosynthetic capabilities (alkaloid production) of an embryogenic cell line and two non-embryogenic C. roseus strains were comparatively examined. Faster cell growth rate was associated with higher alkaloid production in the embryogenic cell line. The kinetics of ajmalicine and serpentine production by the three cell lines is also reported.Keywords
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