ISOLATION AND PROPERTIES OF AN EXOCELLULAR NUCLEASE OF SERRATIA MARCESCENS
- 1 February 1963
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 85 (2) , 273-+
- https://doi.org/10.1128/jb.85.2.273-278.1963
Abstract
The exocellular nuclease of Serratia marcescens (isolated by anion-exchange chromato-graphy on diethylaminoethyl-Sephadex) depolymerized deoxyribo-nucleic-acid, ribonucleic-acid and the polynucleotide which is refractory to pancreatic ribonuclease activity. The enzyme was tentatively classified as a nonspecific phosphodiesterase. Magnesium was essential for activity which was optimal at pH 8.8. The purified enzyme was completely inactivated by heating at 50 C for 15 min.This publication has 12 references indexed in Scilit:
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