Characterization of insertions affecting the expression of the bacterio-opsin gene inHalobacterium halobium
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (5) , 2489-2497
- https://doi.org/10.1093/nar/12.5.2489
Abstract
We have determined the sequence of the inverted repeats and duplicated target DNA of the halobacterial insertion elements ISH2 (520 bp), ISH23 (900 bp) and ISH24 (3000 bp) associated with bacterio-opsin (bop) mutants. ISH2 has a perfect 19 bp inverted repeat (3,5), while both ISH23 and ISH24 have imperfect inverted repeats of 29 bp and 14 bp respectively. ISH23 was shown to be highly homologous to ISH50 (6). Variable lengths of duplicated target DNA are found when ISH2 and ISH23 (ISH50) transpose into different sites. A 550 bp DNA insert ("ISH25") reverts the Bop mutation caused by ISH24. "ISH25" lacks typical structural features of a transposable element. "ISH25" and ISH24 are found adjacent to each other upstream of the bop gene. An identical arrangement of "ISH25" and ISH24 is found in the cccDNA of H. halobium NRC817. Comparative sequence analysis of both areas suggests that the translocation of "ISH25" to the bop gene region occurred by a recombination event.Keywords
This publication has 10 references indexed in Scilit:
- Nucleotide sequence of IS26, a new prokaryotic mobile genetic etementNucleic Acids Research, 1983
- High-frequency spontaneous mutation in the bacterio-opsin gene in Halobacterium halobium is mediated by transposable elements.Proceedings of the National Academy of Sciences, 1983
- Bacterio-opsin mutants of Halobacterium halobiumProceedings of the National Academy of Sciences, 1983
- Structure of the archaebacterial (transposable element ISH50Nucleic Acids Research, 1983
- A transposable element from Halobacterium halobium which inactivates the bacteriorhodopsin gene.Proceedings of the National Academy of Sciences, 1982
- Variant insertion element IS1 generates 8-base pair duplications of the target sequenceNature, 1981
- Studies on Transposition Mechanisms and Specificity of IS4Cold Spring Harbor Symposia on Quantitative Biology, 1981
- A large plasmid from Halobacterium halobium carrying genetic information for gas vacuole formationPlasmid, 1979
- DNA sequence of the transposable element IS1Molecular Genetics and Genomics, 1979
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977