Relationships between in Vivo Survival and (1) Density Distribution, (2) Osmotic Fragility of Previously Frozen, Autologous, Agglomerated, Deglycerolized Erythrocytes

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Abstract
In 39 autotransfusions, significant correlations were observed between (1) density distribution and (2) osmotic fragility and 24‐hour posttransfusion survival of chromium‐labeled human erythrocytes preserved with glycerol using a slow‐freeze agglomeration technic.The temperature of storage and the volume of isotonic saline used to disaggregate the agglomerated red cell mass were critical; optimum results were observed following storage of the preserved cells at –80 C and with disaggregation of the agglomerated red cell mass of each unit with 250 cc of isotonic saline.These data indicate that preserved cells with a marked increase in density and a marked decrease in osmotic fragility had a decreased posttransfusion survival. Within 24 hours following the infusion of preserved cells characterized by a marked increase in density and a marked decrease in osmotic fragility, temporary sequestration of a portion of cells was observed, with release of these cells noted on the third to fourth day following the transfusion.