The small G‐protein ARF1GDP binds to the Gtβγ subunit of transducin, but not to GtαGDP‐Gtβγ
Open Access
- 10 April 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 362 (3) , 286-290
- https://doi.org/10.1016/0014-5793(95)00258-b
Abstract
AlF4 − activates heterotrimeric G‐proteins Gα subunits but not small GDP/GTP‐binding proteins like ARF1. On retinal membranes containing holotransducin (Gt α GDP‐Gt βγ and incubated with ARFGDP, AlF4 − induced Gt α GDP‐AlF4 release and ARFGDP binding, probably to the remaining membrane‐attached Gt βγ. On phospholipid vesicles reconstitued with Gt βγ, ARFGDP bound in proportion to Gt βγ, and was released upon subsequent GtαGDP addition. Thus ARFGDP competes with GtαGDP for binding to Gtβγ, probably through a conserved motif in the ‘α2 helix’ of Gtα and ARF. This motif is found in the C‐terminal helix of PH domains that bind to Gβγ.Keywords
This publication has 28 references indexed in Scilit:
- Modular binding domains in signal transduction proteinsCell, 1995
- Myristoylation of ADP-ribosylation Factor 1 Facilitates Nucleotide Exchange at Physiological Mg 2+ LevelsJournal of Biological Chemistry, 1995
- Structure of the human ADP-ribosylation factor 1 complexed with GDPNature, 1994
- Roles of Lipid Modifications of Transducin Subunits in Their GDP-Dependent Association and Membrane BindingBiochemistry, 1994
- GTPase mechanism of Gproteins from the 1.7-Å crystal structure of transducin α - GDP AIF−4Nature, 1994
- ARF: a key regulatory switch in membrane traffic and organelle structureCurrent Opinion in Cell Biology, 1994
- Structural determinants for activation of the α-subunit of a heterotrimeric G proteinNature, 1994
- Do G protein subunits associate via a three‐stranded coiled coil?FEBS Letters, 1992
- ADP-Ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: A novel role for a GTP-binding proteinCell, 1991
- Fluoroaluminates activate transducin‐GDP by mimicking the γ‐phosphate of GTP in its binding siteFEBS Letters, 1985