Fluoroaluminates activate transducin‐GDP by mimicking the γ‐phosphate of GTP in its binding site
- 28 October 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 191 (2) , 181-185
- https://doi.org/10.1016/0014-5793(85)80004-1
Abstract
Fluoride activation of the cGMP cascade of vision requires the presence of aluminum, and is shown to be mediated by the binding of one AlF‐4 to the GDP/GTP‐binding subunit of transducin. The presence of GDP in the site is required: AlF4 − is ineffective when the site is empty or when GDPßS is substituted for GDP. This sensitivity to the sulfur of GDPßS suggests that AlF4 − is in contact with the GDP. Striking structural similarities between AlF4 − and PO4 −1 lead us to propose that AlF4 − mimics the role of the γ‐phosphate of GTP.Keywords
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