The isolation of molecular genetic markers for the identification of sex.
- 15 September 1993
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 90 (18) , 8324-8326
- https://doi.org/10.1073/pnas.90.18.8324
Abstract
It is difficult to identify sex in many species of eukaryotic organism. This can considerably impede research into their biology. Fortunately, one sex often possesses a unique chromosome termed Y or W. When DNA markers are available for these chromosomes, then sex identification becomes straightforward. We describe a technique that facilitates the isolation of such markers. The procedure makes use of low-stringency PCR to screen randomly selected primers for their ability to amplify sex-specific loci.Keywords
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