Electron Microscopy and Restriction Endonuclease Analysis of RSF2124-pLS11 Composite Plasmid ConstructedIn Vitro
- 1 October 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 42 (10) , 1841-1846
- https://doi.org/10.1080/00021369.1978.10863263
Abstract
The structure of the composite plasmid (pAT2010) between Escherichia coli plasmid RSF2124 (ColEl-Apr) and Bacillus subtilis plasmid pLSll which was constructed in vitro by the “EcoRI-ligase method” was studied. The size distribution of pAT2010 and the heteroduplex molecules between pAT2010 and ColEl were confirmed by electron microscopy. The composite plasmid along with the component plasmid RSF2124 and pLS11 was digested by restriction endonucleases BamHl, SmaI, and SalI. RSF2124, pLSll, and pAT2010 were digested by BamNl into 1, 1, and 2 fragments, respectively. By Smal, the three plasmids were cut into 1, 0, 1 fragment, respectively, but by SalI, they were not digested.This publication has 9 references indexed in Scilit:
- DNA cloning in Bacillus subtilis.Proceedings of the National Academy of Sciences, 1978
- A new restriction endonuclease from Streptomyces albus GJournal of Molecular Biology, 1978
- A New Isolation Method of Plasmid Deoxyribonucleic Acid fromStaphylococcus aureusUsing a Lytic Enzyme ofAchromobacter lyticusAgricultural and Biological Chemistry, 1977
- Purification of cohesive-end-producing restriction endonuclease from Bacillus subtilis GBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1977
- Isolation and characterization of four plasmids from Bacillus subtilisJournal of Bacteriology, 1977
- Molecular cloning and in vitro transcription of Bacillus subtilis plasmid in Escherichia coliMolecular Genetics and Genomics, 1977
- Recombination between bacterial plasmids leading to the formation of plasmid multimersCell, 1976
- Restriction of a bacteriophage of Streptomyces albus G involving endonuclease SalIJournal of Bacteriology, 1976
- The generation of a colE1-Apr cloning vehicle which allows detection of inserted DNAMolecular Genetics and Genomics, 1975