Opening of Ca2+-Dependent K+ Channels by Nordihydroguaiaretic Acid in Porcine Coronary Arterial Smooth Muscle Cells

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Abstract
Effects of nordihydroguaiaretic acid (NDGA) on the Ca(2+)-dependent K+ channel (BK channel) were examined by the patch clamp technique in single smooth muscle cells of porcine coronary artery. The open probability of BK channels in inside-out patches increased by about 30 times at the holding potential of 0 mV, when 10 microM NDGA was added to the bathing solution (pCa 7.0). The effect of NDGA was concentration-dependent in the range of 1-100 microM and partly removed by washout. The enhancement of BK channels by NDGA was not observed when the cytosolic Ca2+ concentration was very low (pCa > 8.5). These results clearly indicate that NDGA possesses a BK channel opening property in coronary arterial myocytes.