Gammaflow: A Completely Automated Radioimmunoassay System

Abstract
The details and initial results of a completely automated on-line radioimmunoassay system, Gamma-flow are reported. This system combines the sample or standard with the labeled ligand and antibody, incubates the samples, separates and determines the amount of labeled ligand bound, and then computes the amount of the unknown sample by comparison to standard curves. The complete operation, at the rate of 20-40 samples/h, can take place in the absence of any human intervention. Automated radioimmunoassays for digoxin, cyclic AMP, cyclic GMP, insulin, angiotensin I and thyroxine are described.