Initial Stages of Mustard Action Upon Influenzal Toxicity and Infectivity.

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Abstract
The problem of residual mustard toxicity which prevented analysis of the early stages of mustard action upon the infective and toxic components of influenza A (PR8) virus was circumvented by detoxification of mustard with Na thiosulfate. Studies of the action of 5 x 10-4 [image] cyclohexyl [bis (beta-chloroethyl)] amine hydrochloride upon virus during the first 30 min. of mustard-virus interaction at 4[degree]C demonstrated a seemingly greater rate of destruction of viral toxicity. The latter activity was lost in the first few minutes of reaction, but the destruction of viral infectivity proved to be a more gradual though progressive process. Concn. of mustard-treated virus by the method of red cell adsorption and elution failed to restore the toxic activity of virus. Despite the apparent completeness of destruction of viral toxicity, studies of viral progeny derived from eggs infected with one ID50 of mustard-treated virus disclosed no permanent loss of this viral attribute.