Isotype analysis of the anti–CENP‐B anticentromere autoantibody: Evidence for restricted clonality
Open Access
- 1 October 1989
- journal article
- research article
- Published by Wiley in Arthritis & Rheumatism
- Vol. 32 (10) , 1315-1318
- https://doi.org/10.1002/anr.1780321019
Abstract
Utilizing the centromere B fusion protein (CENP‐B) and specific, matched monoclonal antiisotype reagents in an enzyme‐linked immunosorbent assay, we found that anti–CENP‐B autoantibodies were skewed to the IgG1 isotype. The overall k:λ light chain ratio was 2:1, although several individual sera showed a strong predominance of one of the light chains. Isoelectric focusing of light chain–skewed sera showed polyclonal patterns. Our findings are consistent with the anti–CENP‐B autoantibody response being a chronic, antigen‐driven response.This publication has 14 references indexed in Scilit:
- Detection of anticentromere antibodies using cloned autoantigen cenp‐BlArthritis & Rheumatism, 1987
- The role of clonal selection and somatic mutation in autoimmunityNature, 1987
- Analysis of anticentromere autoantibodies using cloned autoantigen CENP-B.Proceedings of the National Academy of Sciences, 1987
- Three human chromosomal autoantigens are recognized by sera from patients with anti-centromere antibodies.Journal of Clinical Investigation, 1986
- Subclass restriction of anti-SS-B (La) autoantibodiesClinical Immunology and Immunopathology, 1986
- Inter- and intraclonal diversity in the antibody response to influenza hemagglutinin.The Journal of Experimental Medicine, 1985
- Subclass restriction and polyclonality of the systemic lupus erythematosus marker antibody anti-Sm.Journal of Clinical Investigation, 1985
- Kinetochore components recognized by human autoantibodies are present on mononucleosomes.Molecular and Cellular Biology, 1985
- Synthesis and assembly of human small nuclear ribonucleoproteins generated by cell-free translation.Proceedings of the National Academy of Sciences, 1983
- Autoantibody to centromere (kinetochore) in scleroderma sera.Proceedings of the National Academy of Sciences, 1980