Diagnosis of Acute Typhus Infection Using the Polymerase Chain Reaction
- 1 June 1990
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 590 (1) , 439-444
- https://doi.org/10.1111/j.1749-6632.1990.tb42252.x
Abstract
We used the polymerase chain reaction (PCR) for the diagnosis of an acute rickettsial infection. A primer pair derived from the 17-kDa antigen sequence of Rickettsia rickettsii amplified specifically a 434-bp DNA fragment from the genome of Rocky Mountain spotted fever and endemic and epidemic typhus. The assay was able to detect as few as 30 rickettsiae. Detection of PCR-amplified DNA with a digoxigenin-labeled DNA probe confirmed an acute human infection with Rickettsia prowazekii.Keywords
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