STUDIES ON THE EXTRACELLULAR CULTIVATION OF AN INTRACELLULAR PARASITE (AVIAN MALARIA)
Open Access
- 1 November 1952
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 96 (5) , 465-476
- https://doi.org/10.1084/jem.96.5.465
Abstract
The extracellular survival and development in vitro of the erythrocytic stages of Plasmodium lophurae were favored by the addition to the culture medium of l-malic acid and concentrates rich in coenzyme A. In a concentrated extract of duck erythrocytes supplemented with these two substances in addition to adenosinetriphosphate, sodium pyruvate, and certain other materials of like nature, only 5 to 10 per cent of the extracellular parasites had become abnormal after 3 days of cultivation.Keywords
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