Allotype-specific analysis of anti-(TYR,GLU)-ALA-LYS antibodies produced by Ir-1A high and low responder chimeric mice

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Abstract
To clarify whether lymphoid cell chimerism, with presumed tolerance to H-2 incompatibility, would permit effective cell interactions in response to antigens under Ir [immune response] gene control, bone marrow cell chimeric mice were prepared by using strains differing both for Ig allotype and for high vs. low responsiveness to (T,G)-A-L [poly-L (tyrosine, glutamine)-poly D,L-alanine-poly-L-lysine]. An antigen-specific and allotype-specific antibody assay was used to discriminate the responses produced by high and low responder strain B cells in these chimeras. Lymphoid cell chimerism per se is apparently not sufficient to obviate Ir gene-mediated restrictions in cell interaction.