The estimation of free sugars in skeletal muscle of codling (Gadus callarias) and herring (Clupea harengus)

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Abstract
Ribose in extracts of herring and codling muscles reacts readily with nitrogenous constituents during application to chromatograms. Glucose is stable. Electrolytic de-salting interferes with the estimation of ribose. Sulphonated-polystyrene resins in hydrogen form hydrolyse adenosine at room temperature but the error introduced thereby in muscle analyses is insignificant. Removal of salt and amino nitrogen by suitable mixed ion-exchange resins followed by lyophilizing and quantitative chromatog-raphy gave an estimate of glucose and ribose in codling and herring accurate to [plus or minus]6%.