Improved Sensitivity for in Situ Infrared Identification of Species Separated by Thin-Layer Chromatography Using Programmed Multiple Development
- 1 November 1977
- journal article
- research article
- Published by SAGE Publications in Applied Spectroscopy
- Vol. 31 (6) , 528-530
- https://doi.org/10.1366/000370277774464093
Abstract
A method is described for measuring the infrared spectra of submicrogram quantities of materials separated by thin-layer chromatography (TLC) without removing the sample from the plate. The use of programmed multiple development for the chromatography concentrates each spot of sample on the TLC plate by approximately a factor of 4 compared to conventional TLC. A Fourier transform infrared spectrometer equipped with a mercury cadmium telluride detector enables recognizable spectra to be measured in less than 10 s.Keywords
This publication has 6 references indexed in Scilit:
- Vibrational Spectrometry of Pesticides and Related Materials on Thin Layer Chromatography AdsorbentsApplied Spectroscopy, 1976
- Programmed multiple development : Lateral spot reconcentrationJournal of Chromatography A, 1975
- TLC [thin-layer chromatography]. Programmed multiple developmentAnalytical Chemistry, 1975
- Direct measurement of the infrared spectra of compounds separated by thin-layer chromatographyAnalytical Chemistry, 1975
- Programmed Multiple Development in Thin Layer ChromatographyJournal of Chromatographic Science, 1973
- Infrared Fourier transform spectroscopy in flavor analysis. IV. Spectra of gas chromatography fractionsJournal of Agricultural and Food Chemistry, 1971