Primary sequence and post‐transcriptional modification pattern of an unusual mitochondrial tRNAMet from Tetrahymena pyriformis
- 27 March 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 362 (1) , 24-28
- https://doi.org/10.1016/0014-5793(95)00179-d
Abstract
In a previous investigation of the rDNA region in Tetrahymena pyriformis mitochondrial DNA, we identified a putative tRNAMet gene [Heinonen et al. (1987) J. Biol. Chem. 262, 2879–2887]. On the basis of Northern hybridization analyses, we suggested that this gene is expressed, even though the resulting tRNA would be unusually small and have an atypical dihydrouridine stem‐loop domain. We report here the complete nucleotide sequence and post‐transcriptional modification pattern of this tRNAMet, confirming its predicted primary structure and supporting the view that this structurally aberrant species functions in translation in T. pyriformis mitochondria.Keywords
This publication has 36 references indexed in Scilit:
- Alterations at the 3′‐CCA end of Escherichia coli and Thermus thermophilus tRNAPhe do not abolish their acceptor activityFEBS Letters, 1994
- Nucleoside Modifications Stabilize Mg2+ Binding in Escherichia coli tRNAVal: An Imino Proton NMR InvestigationBiochemistry, 1994
- Structural compensation in atypical mitochondrial tRNAsNature Structural & Molecular Biology, 1994
- A Novel Modified Nucleoside Found at the First Position of the Anticodon of Methionine tRNA from Bovine Liver MitochondriaBiochemistry, 1994
- Modification of tRNA as a regulatory deviceMolecular Microbiology, 1993
- Relaxation of a transfer RNA specificity by removal of modified nucleotidesNature, 1990
- TRANSFER RNA MODIFICATIONAnnual Review of Biochemistry, 1987
- The formation of several mitochondrial enzymes during the cell cycle in heat-synchronized Tetrahymena pyriformis STExperimental Cell Research, 1978
- ACCUMULATION OF REPLICATIVE INTERMEDIATES OF MITOCHONDRIAL DNA IN TETRAHYMENA PYRIFORMIS GROWN IN ETHIDIUM BROMIDEThe Journal of cell biology, 1974
- Comparative studies on mitochondrial and cytoplasmic ribosomes of Tetrahymena pyriformisJournal of Molecular Biology, 1970