Semiconductor nanocrystal probes for human metaphase chromosomes
Open Access
- 1 February 2004
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 32 (3) , 28e-28
- https://doi.org/10.1093/nar/gnh024
Abstract
To improve signal stability and quantitation, an optically stable, novel class of fluorophore for hybridization analysis of human metaphase chromosomes is demonstrated. Detection of hybridization sites in situ was based on fluorescence from streptavidin‐linked inorganic crystals of cadmium selenide [(CdSe)ZnS]. Fluorescence of nanocrystal fluorophores was significantly brighter and more photostable than organic fluorophores Texas Red and fluorescein. Thus, semiconductor nanocrystal fluorophores offer a more stable and quantitative mode of fluorescence in situ hybridization (FISH) for research and clinical applications.Keywords
This publication has 12 references indexed in Scilit:
- Statistical Aging and Nonergodicity in the Fluorescence of Single NanocrystalsPhysical Review Letters, 2003
- Lighting Up Cells with Quantum DotsBioTechniques, 2003
- Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dotsNature Biotechnology, 2002
- In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid MicellesScience, 2002
- Nanocrystal targeting in vivoProceedings of the National Academy of Sciences, 2002
- Quantum-dot-tagged microbeads for multiplexed optical coding of biomoleculesNature Biotechnology, 2001
- Hydroxylated Quantum Dots as Luminescent Probes for in Situ HybridizationJournal of the American Chemical Society, 2001
- Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic DetectionScience, 1998
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- A general method for isolation of high molecular weight DNA from eukaryotesNucleic Acids Research, 1976