Glycosylation, ADP-ribosylation, and methylation of Tetrahymena histones
- 18 January 1983
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 22 (2) , 484-489
- https://doi.org/10.1021/bi00271a035
Abstract
Some of the postsynthetic modifications that occur in macronuclear histones from T. thermophila were examined. When purified macronuclei are incubated with [32P]NAD+, histones H1, H2A, H2B and H3 are ADP-ribosylated. Histones H1, H2A, H2B and H3 contain fucose and mannose residues as evidenced by the incorporation of [3H]fucose and by the specific binding to these proteins of gorse seed lectin and concanavalin A. Finally, studies on incorporation of methyl groups into histones show that histone H2A, together with the related nonhistone protein A24, is methylated in Tetrahymena.This publication has 18 references indexed in Scilit:
- In vitro splicing of the ribosomal RNA precursor in nuclei of tetrahymenaCell, 1980
- Transcriptionally active mononucleosomes from trout testis are heterogeneous in composition.Journal of Biological Chemistry, 1979
- Nuclear protein modification and chromatin substructure. 3. Relationship between poly(adenosine diphosphate) ribosylation and different functional forms of chromatinBiochemistry, 1979
- Nuclear protein modification and chromatin substructure. 2. Internucleosomal localization of poly(adenosine diphosphate-ribose) polymeraseBiochemistry, 1978
- Properties of the Complex between Histone H1 and Poly(ADP‐ribose) Synthesised in HeLa Cell NucleiEuropean Journal of Biochemistry, 1977
- The identification of membrane glycocomponents in polyacrylamide gels: A rapid method using 125I-labeled lectinsAnalytical Biochemistry, 1977
- Isopeptide linkage between nonhistone and histone 2A polypeptides of chromosomal conjugate-protein A24.Proceedings of the National Academy of Sciences, 1977
- Poly(adenosine diphosphate-ribose) polymerase: the distribution of a chromosome-associated enzyme within the chromatin substructureBiochemistry, 1977
- Purification and characterization of the histones associated with the macronucleus of TetrahymenaBiochemistry, 1976
- Sites of in vivo histone methylation in developing trout testis.Journal of Biological Chemistry, 1975