Synchrotron hard x-ray microprobe: Fluorescence imaging of single cells
- 24 May 2001
- journal article
- research article
- Published by AIP Publishing in Applied Physics Letters
- Vol. 78 (22) , 3544-3546
- https://doi.org/10.1063/1.1366362
Abstract
Imaging of trace elements in single cells was achieved by synchrotron-induced x-ray fluorescence (SXRF) in the hard x-ray range. Monochromatic and “pink” excitations at 14 keV were used with compound refractive lenses resulting in a 1×10 beam size. The experiment shows that SXRF is well suited for microanalysis of freeze-dried cells, and demonstrated high accuracy in quantitative imaging of trace element in cells treated with pharmacological doses of an iodine-labeled anticancer drug.
Keywords
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