Determination of Endogenous Indole-3-Acetic Acid in Plagiochila arctica (Hepaticae)

Abstract
Endogenous IAA was found in axenically cultured gametophytes of the leafy liverwort, P. arctica Bryhn et Kaal, by high-performance liquid chromatography with electrochemical detection. Identification of the methylated auxin was confirmed by gas chromatography-mass spectrometry. Addition of 57 .mu.M IAA to cultures increased relative production of ethylene. This is the 1st definitive (gas chromatography-mass spectrometry) demonstration of the natural occurrence of IAA in a bryophyte.