Identification and Quantitative Analysis of Indole-3-Acetyl-l-Aspartate from Seeds of Glycine max L.
- 1 September 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 70 (3) , 749-753
- https://doi.org/10.1104/pp.70.3.749
Abstract
Indole-3-acetyl-L-aspartate (IAAsp) was isolated from seeds of G. max. L. cv. Hark and its identity established by its chromatographic performance and its mass spectral fragmentation. Following acid hydrolysis, the aspartate moiety was the L-enantiomer by reverse phase high performance liquid chromatographic retentiom time of the bisethyl ester derivatized with 2,3,4,6-tetra-O-acetyl-.beta.-D-glycopyranosyl isothiocyanate. Isotope dilution analysis using [14C]IAAsp as internal standard showed that soybean seed contained 10 .mu.mol/kg IAAsp and this accounted for 50% of the total IAA of the seed.Keywords
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